87% in exposure to cytotoxic drugs, 33.93% in pH sensing, and 30.81% in carbon source responses) were not classified by the MIPS annotation

(Fig. 1). Growth of T. rubrum in a keratinocyte serum-free medium (Library 1) revealed DMXAA 207 novel genes (Table 1; Additional file 2) in comparison to the T. rubrum sequences deposited in public databases, which include an EST collection that was previously generated during the growth of T. rubrum in Sabouraud liquid medium [14]. This suggests that the expression of these 207 novel genes is nutrient-dependent. Functional grouping of these genes, which were identified on the basis of their ESTs, revealed their possible involvement in various cellular processes such as basic metabolism, conidial germination, and hyphal growth, among other functions (see Additional file 2). Figure 1 T. rubrum unigenes functional categorization, according to MIPS. The unigenes were grouped in four different stimuli. Challenging

T. rubrum with cytotoxic drugs Numerous signal-transduction pathways are used selleckchem by fungi to sense and overcome the toxic effects of antifungal drugs [17]. Our aim in this study was to identify metabolic events that occur during the initial stages of drug exposure; therefore, we created an EST collection by challenging the dermatophyte T. rubrum with cytotoxic drugs, including most of the antifungals used in medical practice. These drugs, which belong to the azole and allylamine/thiocarbamate classes, were fluconazole (FLC), imazalil (IMZ), itraconazole (ITRA), ketoconazole (KTC), tioconazole

Inositol monophosphatase 1 (TIO), and terbinafine (TRB). All of these compounds inhibit the biosynthesis of ergosterol. T. rubrum was also challenged with the following cytotoxic drugs: amphotericin B (AMB), griseofulvin (GRS), benomyl (BEN), undecanoic acid (UDA), cycloheximide (CHX), chloramphenicol (CAP), acriflavin (ACR), ethidium bromide (EB), and 4-nitroquinoline 1-oxide (4NQO) [18–20]. Approximately 300 unigenes were identified in these experiments and only 70 of these were exclusive to drug challenge (Additional file 2). Drug exposure induced the transcription of several multidrug resistance genes, as previously reported in studies in which T. rubrum was exposed to sub-inhibitory levels of KTC, AMB, or other drugs [21, 22]. One of these genes [GenBank: FE526598] encodes a putative multidrug resistance protein (MDR) that accumulates in the mycelia when the organism is independently exposed to various cytotoxic agents. Overexpression of this gene has been previously reported in the myceliaof T. rubrum exposed to the antimycotic agents ACR, GRS, ITRA, or FLC [23]. Disruption of this gene increased the susceptibility of the mutant strain to TRB in comparison with the control, suggesting that this transporter modulates T. rubrum drug susceptibility [23]. Some of the ESTs that were JAK inhibitor overexpressed in the mycelia of T.