haemolyticus JCSC1435 (locus SH0122) orf42 43522-44046 DUF3267 type protein 100%, S. haemolyticus JCSC1435 (locus SH0123) orf43 44998-44120 Hypothetical protein, similar to cobalamin synthesis related protein CobW 100%, S. haemolyticus JCSC1435 Nutlin-3a mouse (locus SH0124) orf44 45625-46248 Hypothetical protein, similar to Zn-binding lipoprotein AdcA 100%, S. haemolyticus JCSC1435 (locus SH0125) a Positions are according to GenBank accession no. JQ764731. b GenBank accession no.: S. aureus LGA251 (FR821779), S. aureus JCSC6943 (AB505628), S. aureus JCSC6945 (AB505630), S. aureus M10/0061 (FR823292), S. aureus MSHR1132 (FR821777), S. carnosus TM300 (AM295250), S. epidermidis ATCC 12228 (AE015929), S. epidermidis RP62a
(CP000029), S. haemolyticus JCSC1435 (AP006716), S. saprophyticus ATCC
15305 (AP008934), Oceanobacillus iheyensis HTE831 (BA000028), S. aureus plasmid SAP099B (GQ900449), S. aureus plasmid EDINA (AP003089), S. epidermidis plasmid SAP105A (GQ900452), S. xylosus plasmid pSX267 (M80565). c Closest matches of MGE (IS431 and ISSha1) and genes belonging to the mec complex are not listed as there are many identical matches. d Truncated by IS431 with 19 bp of the 3′ end missing and the read frame extending into IS431. e The tnpA of IS431 was terminated prematurely due to internal point mutation. mecA is bracketed by two copies of IS431 flanking by an 8-bp direct repeat sequence WCH1 had a class C1 mec gene complex composed of mecA, mecR1Δ truncated by the insertion of the insertion sequence IS431, several other genes and another Cell Cycle inhibitor copy of IS431 downstream of mecA with the two copies of IS431 at the same orientation (www.selleckchem.com/products/crenolanib-cp-868596.html Figure 1). The class C1 mec gene complex is also present in SCCmec types VII and X of Staphylococcus aureus and several unnamed types of SCCmec in coagulase-negative staphylococci (CoNS) [9]. An 8-bp identical sequence (CTTTTTGC; Figure 1) was identified flanking the two copies of IS431. The 8-bp DR was part of the spacer sequence between arsR (encoding an arsenical resistance operon repressor) and copA (encoding a copper-exporting ATPase). The presence of a direct repeat (DR) suggested that the two copies of IS431
might have formed a composite transposon with the potential to mediate the mobilization of mecA into different genomic locations. This mecA-carrying IS431-formed composite transposon was designated Tn6191 Liothyronine Sodium according to the transposon database (http://www.ucl.ac.uk/eastman/tn/). Composite transposons formed by IS431 generating 8-bp AT-rich DR on insertion have been seen before, such as Tn6072 carrying ccrC and the aminoglycoside resistance determinant aacA found in a ST239 S. aureus[10]. Two copies of IS431 have also been found to mediate the transposition of plasmids pUB110 encoding bleomycin resistance [11] and pT181 encoding tetracycline and mercury resistance [12]. However, Tn6072 and other IS431-formed composite transposons do not contain mecA.