0, 5.4, and 4.1 large caliber vessels pr. 1000 ��m2 tissue in the ALDHhiLin-, ALDHloLin- and PBS treated groups, respectively (95% confidence interval [5.0-7.0], [4.4-6.5], never [3.3-5.0]; Table Table1).1). We found a significant increase in capillary density in the ALDHhiLin- treated group as compared to the PBS treated group at four weeks post transplantation (p = 0.011 versus PBS; Table Table1).1). Although the ALDHloLin- treated group was not significantly different from the PBS treated group, we noted a tendency toward an intermediate improvement in vascular density in the ALDHloLin- treated groups. Using the Hadis method to identify outliers in multivariate data[21] with a 95% significance level eliminated two high power fields in the PBS treated groups and one outlier in the ALDHhiLin- treated group.

Between group comparison after elimination of outliers revealed that both the ALDHhiLin- treated and the ALDHloLin- treated groups were significantly different from the PBS treated group (p = 0.001 and p = 0.031, respectively). Figure 6 Vascular density in the infarct zone of NOD/SCID ��2m null mice with AMI four weeks after transplantation of ALDHlo Lin- or ALDHhi Lin- sorted human UCB cells. NOD/SCID ��2m null mice with AMI were transplanted with ALDHloLin- or ALDHhi … Table 1 Mean vascular density in the infarct zone of NOD/SCID ��2m null mice with AMI four weeks post transplant of PBS, ALDHlo Lin- or ALDHhi Lin- sorted human UCB cells Discussion In the current studies we have adapted the LAD occlusion model of AMI to immune deficient NOD/SCID and NOD/SCID ��2m null mice.

We used this model to evaluate the global engraftment potential of purified human UCB cell populations as well as the distribution, engraftment, and regenerative potential for the infarcted heart. We first used fluorescent nanoparticle labeling to trace the donor cell distribution to various organs, including the infarcted myocardium, following IV injection. We have recently documented that sorting of the labeled cells is essential to avoid infusing large numbers of unbound nanoparticles[17]. Non-cell mediated splenic sequestering of fluorescent nanoparticles was indeed pronounced in our previous report when control NOD/SCID ��2m null mice received free 750 nm fluorescently conjugated Feridex nanoparticles[17].

The fluorescent intensities found in the NOD/SCID mice transplanted with QD655 labeled cells in the present study may thus include both cell specific and unspecific non-cell mediated fluorescence. Our present results from animals transplanted with 750 nm Feridex labeled cells sorted prior to infusion, Carfilzomib however, confirm a significant distribution of labeled donor cells to the infarcted tissue in the absence of nonspecific signal from free nanoparticles. We have previously found a labeling efficiency between 28% and 40% with fluorescently conjugated Feridex nanoparticles, depending of the purification method[17].