001, One-way-ANOVA with Tukey’s posthoc test). Further analysis of
TI mucosal tissue from IBD patients revealed increased inflammatory (IL-17+) (IBD, 1.1 ± 0.4%; control, 0.23 ± 0.04%, n = 3, P < 0.05, unpaired Student's BAY 80-6946 research buy t-test) and CD8+ regulatory (FoxP3+CD25Hi) T cells (IBD, 0.95 ± 0.20%; control, 0.16 ± 0.06%, n = 3, P < 0.01). This illustrates the efficacy of these methods in analysing healthy and inflamed environments and that IBD mucosa harbours distinct immune populations. Conclusion: Results thus far indicate these methods are effective for T cell characterisation within intestinal tissue of healthy and IBD patients. Increased T cells were present in the healthy terminal ileum, the most common site of IBD Smoothened Agonist concentration inflammatory lesions, compared with the colon.
As expected inflammatory T cells were increased in inflamed colonic tissue compared with healthy, but surprisingly, regulatory T cells were also increased in the inflammed colonic tissue. Further study will incorporate analysis of SpA patients, completing study of the pathophysiological crossover between IBD and SpA. Knowledge of the presence and function of innate and adaptive immune cell populations will provide insight into the linkages between IBD and SpA and how the immune balance has been altered to favour disease progression. Key Word(s): 1. IBD; 2. T cells; 3. Colitis; 4. Inflammation; Presenting Author: NAZRI MUSTAFFA Additional Authors: IDA NORMIHA HILMI, APRILC ROSLANI, KHEAN LEE GOH Corresponding Author: NAZRI MUSTAFFA Affiliations: University of Sydney; University of Malaya Objective: It is a recognized fact that patients MCE公司 with inflammatory bowel disease (IBD) are at an increased risk of developing gastrointestinal-related malignancies or extraintestinal solid tumors. Here we present a case of
an IBD patient on biologic therapy, who subsequently went on to develop a rapidly advancing colonic malignancy. Methods: Mrs SNH was initially diagnosed as having pancolonic Crohn’s disease with a rectovaginal fistula in 2007 at the age of 28. She had a partial response to prednisolone and subsequently received three doses of infliximab (combined with azathioprine). Her condition however did not improve. Results: After defaulting follow-up for two years, she again presented in November 2012 with progressive abdominal distension. CT-scan was suggestive of subacute bowel obstruction, but no malignancy was seen. She was not keen for surgery and was treated conservatively with adalimumab and total parenteral nutrition. Her abdominal distension worsened, and a repeat CT-scan abdomen revealed a cecal tumor with right ovarian involvement and metastatic peritoneal deposits. She then underwent an extended right hemicolectomy and a right sweeping oophorectomy. Post-operative specimen revealed the tumour to be a cecal mucinous adenocarcinoma. She is now undergoing chemotherapy. Conclusion: Colonic carcinoma is a rare but well recognized complication of Crohn’s disease.