05) in coculture with F. succinogenes S85 than in monoculture. Significantly higher growth (P < 0.05) of strain R-25 in coculture was also observed at end point. Although the growth of F. succinogenes

S85 in coculture with strain R-25 was lower (P < 0.05) than that for F. succinogenes S85 monoculture after 48 h of incubation, higher copy number (P < 0.05) was observed in coculture with strain R-25 than in monoculture after 96 h of incubation. In monoculture containing rice straw as a carbon source, Erastin strain R-25 produced d-lactate, acetate, l-lactate, and succinate, meanwhile F. succinogenes S85 released succinate, acetate, propionate, and d-lactate (Supporting information, Table S1). Among these organic acids, d-lactate

and succinate were the main metabolites produced by strains R-25 and F. succinogenes S85, respectively; therefore, only d-lactate and succinate production are shown (Table 2). Lactate production in monoculture of strain Bleomycin ic50 R-25 was 2.0 μmol mL−1 of culture at 48 h and did not increase over the period of 48–96 h. In contrast, lactate production in coculture of strain R-25 with F. succinogenes S85 increased continuously up to 96 h. In particular, there was a marked increase from 48 to 96 h. Although succinate concentration at 96 h was similar between monoculture and coculture, the rate of production until 48 h was greater in coculture, producing significantly higher concentration at 48 h (P < 0.05). Growth of strain R-25 in the supernatant of F. succinogenes S85 culture (OD660 nm = 0.10) was comparable with that in cello- or xylo-oligosaccharide medium (OD660 nm = 0.12). Histone demethylase Intracellular and extracellular enzyme activities of strain R-25 on various media are shown in Table 3. CMCase activity of strain R-25 was lower than 1 nmol min−1 mL−1 culture, irrespective of the media and enzyme fractions. On the other hand, intracellular xylanase activity was significantly higher (P < 0.05) in the supernatant of F. succinogenes S85 culture (6.8 nmol min−1 mL−1

culture) and xylooligosaccharide medium (2.7 nmol min−1 mL−1 culture). However, xylanase activity was low or negligible in the extracellular fraction. DM digestion of rice straw and concentration of major organic acids in the culture of strain R-25, F. succinogenes S85, S. ruminantium S137, and in combination are shown in Table 4. DM digestion was significantly higher in coculture than in monoculture of F. succinogenes S85, and the highest digestion was observed in triculture (P < 0.05). The major organic acids in monocultures of strains R-25, F. succinogenes S85, and S. ruminantium S137 were d-lactate, succinate, and propionate, respectively. In coculture of strains R-25 and F. succinogenes S85, succinate, d-lactate, and acetate were detected. The main acids in coculture of F. succinogenes S85 and S. ruminantium S137 were propionate and acetate. The main products in the triculture were also propionate and acetate.