Although some of the biochemical and hematological Ro 61-8048 manufacturer parameters were statistically different between the test and negative control group, these differences were not biologically significant. Figure 3 Changes of the blood biochemical data of rats treated with C-dots. The rats were treated with C-dots at doses of 0.2, 2, and 20

mg/kg BW in 1, 3, 7, and 28 days. (A) GPT, (B) GOT, (C) urea, (D) cholesterol, (E) TG, (F) blood glucose, (G) Cr, (H) total protein, and (I) albumin. The organs of the rats injected with C-dots at the highest dose of 20 mg/kg BW were harvested for histopathological analysis. These organs included the heart, liver, spleen, stomach, kidneys, lungs, brain, stomach, intestines, ovaries, and testes. As shown in Figure 4, no obvious organ damage was noticed. Likewise, no apparent histopathological abnormality or lesion in the test groups was observed. The size and structure of the cardiac muscle fibers in the test group were uniform and normal. There was no steatosis, MM-102 necrosis, or hydropic degeneration in the exposed Cilengitide clinical trial hepatic sections. The structure of the liver lobule was normal, with few collagen fibers located in the portal area and central vein. The splenic capsule was complete, and the red and white pulps

were clear. The lung structures were normal and no inflammation was Org 27569 found. In the sections of the kidneys, the glomerular structure was easily distinguished. No bleeding, ulcer, or abnormal differentiation was observed in the gastric mucosa. Figure

4 Results of histopathological analyses of rats. The rats were treated with C-dots at the dose of 20 mg/kg BW at 30 days. No significant difference was found between the weights of the major organs (liver, spleen, kidney, ovaries, and testes) between the test groups (both female and male) and negative control group (P > 0.05), as shown in Table 5. Table 5 Diversification of rat major organ weight Gender Dose Body weight (g) Liver Spleen Kidney Ovarian/testis       Wet weight (g) Liver/body (%) Wet weight (g) Spleen/body (%) Wet weight (g) Kidney/body (%) Wet weight (g) Organs/body (%) Female Negative control 235.9 ± 17.2 6.74 ± 0.66 2.86 ± 0.22 0.60 ± 0.07 0.26 ± 0.03 1.78 ± 0.14 0.75 ± 0.05 0.24 ± 0.07 0.10 ± 0.03   Low 235.8 ± 12.8 6.92 ± 0.53 2.94 ± 0.20 0.59 ± 0.04 0.25 ± 0.02 1.70 ± 0.12 0.72 ± 0.03 0.27 ± 0.05 0.12 ± 0.02   Middle 234.9 ± 13.9 6.61 ± 0.53 2.83 ± 0.30 0.59 ± 0.03 0.25 ± 0.01 1.71 ± 0.09 0.73 ± 0.05 0.25 ± 0.05 0.11 ± 0.02   High 230.8 ± 20.6 6.67 ± 0.90 2.88 ± 0.22 0.56 ± 0.07 0.24 ± 0.02 1.76 ± 0.12 0.76 ± 0.03 0.26 ± 0.06 0.11 ± 0.02 Male Negative control 362.5 ± 22.7 12.52 ± 1.94 3.44 ± 0.34 0.91 ± 0.14 0.25 ± 0.04 2.79 ± 0.25 0.77 ± 0.05 3.13 ± 0.13 0.86 ± 0.03   Low 352.9 ± 17.8 11.21 ± 1.05 3.18 ± 0.