Ascostromata visible as minute black

Ascostromata visible as minute black FRAX597 in vivo dots or papilla on host tissue, semi-immersed to erumpent under epidermis, individually globose to subglobose, solitary or clustered, longitudinal

axis vertical to the host surface. Ostiole central, circular, papillate. Peridium of locules two-layered, outer layer composed of brown to dark brown, Anlotinib manufacturer thick-walled cells of textura angularis, inner layer composed of hyaline thin-walled cells of textura angularis. Pseudoparaphyses hyphae-like, numerous, septate, slightly constricted at septum. Asci 8−spored, bitunicate, fissitunicate, clavate to cylindro-clavate, short pedicellate, apically rounded with an ocular chamber. Ascospores hyaline, aseptate, ellipsoidal to fusiform, thick-walled. Pycnidial aggregates morphologically

indistinguishable from ascomatal aggregates. Pycnidia globose and non-papillate to pyriform, with a short, acute papilla; pycnidium a locule created within stromal tissue; pycnidial wall not differentiated from surrounding tissue. Conidiogenous cells holoblastic, hyaline, subcylindrical, proliferating percurrently with 1–2 proliferations and periclinical selleck kinase inhibitor thickening. Conidia ellipsoidal with apex round and base flat, hyaline, aseptate, becoming light brown and 1–2 septate with age (asexual morph description follows Pennycook and Samuels 1985). Notes: Neofusicoccum was introduced for an asexual morph which occurs with a “Dichomera”-like synanamorph by Crous et al. (2006). They considered that the name is more informative of the morphological state. Most of the species of the genus had previously been treated as Fusicoccum, and Crous et al. (2006) proposed new combinations for 13 species based on the sequence data from cultures. Pennycook and Samuels (1985) listed Fusicoccum parvum as the asexual morph when they described Botryosphaeria parvum (= Neofusicoccum

next parvum). In the present study we found the sexual morph of Neofusicoccum parvum, the type species of the genus, on a branch of Linum usitatissimum. The isolate clustered with the type strain of N. parvum with 100 % bootstrap support (Fig. 1). Morphologically our collection is identical to the original description of N. parvum. Generic type: Neofusicoccum parvum (Pennycook & Samuels) Crous, Slippers & A.J.L. Phillips Neofusicoccum parvum (Pennycook & Samuels) Crous, Slippers & A.J.L. Phillips, Stud. Mycol. 55: 248 (2006) MycoBank: MB500879 (Fig. 26) Fig 26 Neofusicoccum parvum on dead branch of Linum usitatissimum (MFLU 11–0220). a Ascostromata on host tissue. b Section through ascostroma. c Section through peridium. d Pseudoparaphyses. e Asci with pseudoparaphyses. f−i Asci. j−k Ascospores. Scale bars: a = 500 μm, b = 200 μm, c−d = 20 μm, e−i = 30 μm, j−m = 10 μm ≡ Fusicoccum parvum Pennycook & Samuels, Mycotaxon 24: 455 (1985) ≡ Botryosphaeria parva Pennycook & Samuels, Mycotaxon 24: 455 (1985) Saprobic on dead branch.

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