Inourstudy,IL16 wasdown regu lated and participated in T cell regulation with IL7R in cold pattern RA individuals but not in heat pattern RA individuals. EIF4A2 is really a gene for a single from the protein synthesis initiation variables concerned inside the binding of mRNA to your ribosome. One study indicated that EIF4A2 controls mRNA specic trans lation as well as the protein synthesis rate in pancreatic beta cells and that EIF4A2 is down regulated by large glucose levels in rat beta INS832/13 cells. In our research, based on the PPI evaluation, EIF4A2 like a seed gene was down regulated in cold pattern RA patients and was related to the regulation of translation and cell biosynthetic processes, however it was not altered in heat pattern RA individuals.
For TCM heat pattern RA individuals, closely related path ways, which include inhibitor Raf Inhibitors antigen processing and presentation, endo cytosis, MAPK signaling, RNA degradation, hematopoietic cell lineage, complement and coagulation cascades, mTOR signaling, adipocytokine signaling, regulation of autophagy, hypertrophic cardiomyopathy, and insulin signaling, had been discovered. The dierentially expressed main genes have been CAMP, PRKAA1, HSPA1A, HSPA8, and LSM6 in heat pat tern RA sufferers. CAMP is surely an antimi crobial protein identified in specic granules of polymorphonu clear leukocytes. The cathelicidin family is cha racterized by a conserved N terminal cathelin domain along with a variable C terminal antimicrobial domain that can be re leased in the precursor protein just after cleavage by protei nases. In our study, CAMP was up regulated 2.
33 fold and participated in T cell regulation and cell proliferation in TCM heat pattern RA individuals but not in cold pattern RA patients. PRKAA1 Aprepitant belongs to the ser/thr protein kinase family members. It is the catalytic subunit from the 5 prime AMP activated professional teinkinase. AMPK regulatestheactivitiesofanum ber of key metabolic enzymes by phosphorylation and protects cells from stresses that bring about ATP depletion by swit ching o ATP consuming biosynthetic pathways. It par ticipated within the mTOR signaling pathway, the adipocytokine signaling pathway, the regulation of autophagy, hypertrophic cardiomyopathy, and the insulin signaling pathway on the KEGG pathway in heat pattern, RA patients but not in cold pattern RA patients. According to the PPI evaluation, PRKAA1, as being a seed gene, was up regulated and participated in fatty acid metabolism in TCM heat pattern RA patients.
HSPA1A is a 70kDa heat shock protein plus a member with the heat shock protein 70 relatives. Together with other heat shock proteins, this protein stabilizes present proteins against aggregation and mediates the folding of newly translated proteins during the cytosol and in organelles. It’s also concerned within the ubiquitin proteasome pathway via interaction with

the AU rich element RNA binding protein one.