RESULTS: Concentrated phosphoric acid was the ultimate cellulose solvent for dissolution
pretreatment since 5% w/w cellulosic fabric can be completed dissolved at 50 degrees C. After regeneration in water, the cellulosic precipitate was subjected to cellulase hydrolysis, resulting in at least 4-fold enhancement of saccharification rate and reducing sugars yield. The colored saccharification products can be utilized by G. xylinus to produce BC, approximately 1.8 g L(-1) Rapamycin BC pellicle was obtained after 7 days static cultivation.
CONCLUSION: Dyed and blended waste fabric can be pretreated effectively by dissolution to produce fermentable sugars by cellulase hydrolysis. Dissolution pretreatment can expose the dyed or polyester fiber covered digestible cellulosic fibers to cellulase and leads to check details a significant enhancement of saccharification yield. The colored saccharification products have no significant inhibiting effect on the fermentation activity of G. xylinus for BC production. (C) 2010 Society of Chemical Industry”
“OBJECTIVE: To estimate the clinical significance of serum anti-human papillomavirus (HPV)
antibodies and high-risk cervical HPV DNA in cervical neoplasia.
METHODS: The study population comprised patients who were histopathologically diagnosed with cervical intraepithelial neoplasia (CIN) 1 (n=64), CIN 2 and 3 (n=241), cervical cancer (n=170), and normal control participants (n=975). Cervical HPV DNA tests were performed through nucleic MX69 manufacturer acid hybridization assay tests, and serum anti-HPV 16 and 18 antibodies were measured by competitive immunoassay. The associations of HPV DNA and anti-HPV antibodies were evaluated with demographic characteristics and compared according to the levels of disease severity. Anti-HPV antibodies were also
investigated with clinicopathologic parameters, including survival data.
RESULTS: Among various demographic characteristics, factors involving sexual behavior had a higher tendency of HPV DNA positivity and HPV seropositivity. Human papillomavirus DNA mean titer and positivity were both increased in patients with cervical neoplasia compared with those with normal control participants, but there was no statistical difference among types of cervical neoplasia. Serum anti-HPV 16 antibodies were also able to differentiate cervical neoplasia from a normal control participant and furthermore distinguished CIN 1 from CIN 2 and 3 (odd ratio 2.87 [1.43-5.78], P=.002). In cervical cancer, HPV 16 seropositivity was associated with prolonged disease-free survival according to the univariable analysis (hazard ratio=0.12 [0.01-0.94], P=.044).
CONCLUSION: Serum anti-HPV 16 antibodies can distinguish cervical neoplasia from a normal control and has the advantage of identifying high-grade CIN.