Stromata when dry (1.3–)1.5–3.3(–5.1) × (0.9–)1.2–2.4(–3.2) mm, (0.5–)0.6–1.4(–2.0) mm thick (n = 20), solitary, less commonly gregarious

or aggregated in small numbers, erumpent from bark, centrally attached, typically on a white, columnar or pulvinate, compact mycelial Selleckchem Bucladesine base, with upper fertile part free and often incurved at the margin, pulvinate or semiglobose; outline roundish, oblong or irregularly lobed. Surface smooth, slightly tubercular or rugose, glabrous or slightly downy or whitish floccose. Ostiolar dots (30–)47–106(–165) μm (n = 90) diam, numerous, densely disposed, well-defined when mature, often confluent, convex to papillate, orange to nearly red. Stroma colour bright orange (bright yellow surface, orange dots), 5–6AB5–8. White inside. Spore deposits white. Dry stromata instantly transparent and discoloured to pale yellowish after addition of 3% KOH on a slide. Rehydrated buy Duvelisib stromata ca 30% larger than dry, semiglobose, light yellowish, discoloured, white with pale orange-ochre ostiolar dots; no change noted after addition of 3% KOH. Stroma anatomy: Ostioles (67–)75–98(–116) μm long, plane or projecting to 15(–30) μm,

(28–)30–45(–60) μm wide at the apex (n = 31), typically only periphysate, less commonly with some clavate marginal cells to 6 μm wide at the apex; often ostiolum and stroma cortex projecting to 50–90 μm. Perithecia (160–)200–250(–290) × (90–)130–200(–215) μm (n = 31), flask-shaped, ellipsoidal or subglobose, mostly crowded, 7–8 per mm stroma length. Peridium (11–)13–19(–21) μm (n = 31) thick at the base, (5–)10–16(–18) μm (n = 31) at the sides, pale yellowish. Cortical layer (16–)20–30(–35) μm (n = 30) thick, a dense, subhyaline to pale yellowish t. angularis of isodiametric or oblong, thick-walled cells (2.5–)4–8(–11) × (2.5–)3–5(–7) μm in face view and in vertical section (n = 62). Cortex of young stromata covered by a reticulum of thick-walled OSBPL9 hyaline hyphae, when mature remaining as hairs (4–)8–24(–35) × (2.5–)3.0–4.5(–5.5) μm (n = 30), cylindrical, straight or curved, simple or branched, hyaline, thin-walled. Subcortical tissue a t.

intricata of thin-walled hyaline hyphae (2.0–)2.5–4.5(–7.0) μm (n = 30) wide. Subperithecial tissue a t. epidermoidea–intricata of thick-walled hyaline cells (4–)7–30(–58) × (4–)7–14(–22) μm (n = 30) and hyphae (3.5–)6–13(–19) μm (n = 30) wide. Non-attached base a loose or dense t. intricata of hyaline or yellowish, thick-walled hyphae (2.0–)2.5–4.5(–6.0) μm (n = 30) wide. Asci (85–)100–130(–150) × (5.0–)5.5–6.2(–7.0) μm; stipe (5–)13–28(–41) μm long (n = 60); croziers present. Ascospores hyaline, verruculose; cells dimorphic; distal cell (4.0–)4.5–5.7(–6.7) × (3.7–)4.0–4.5(–5.0) μm, l/w (1.0–)1.1–1.4(–1.7) (n = 110), subglobose or wedge-shaped; proximal cell (4.5–)5.2–6.5(–7.8) × (3.0–)3.3–4.0(–4.5) μm, l/w (1.2–)1.4–1.8(–2.2) (n = 110), oblong or subglobose; size increasing with maturation.