The analytes and internal standard were isolated from acidified plasma using liquid-liquid extraction (LLE). The organic extracts were evaporated, reconstituted in mobile phase and injected into the HPLC-MS/MS system. The analytes were chromatographed on a XB-C8 analytical column and MS-MS detection was performed on an electrospray ionization tandem mass spectrometer operated in multiple reaction monitoring (MRM) mode. Precursor. product combinations of m/z 369.9 -> 177.0, 286.3 -> 201.1 and 285.6 -> 193.1 were used to quantify curcumin, piperine and the internal standard (IS), respectively. The assay was validated

in the concentration range of 1.0 – 100.0 ng/ml for curcumin and 0.5 – 800.0 ng/ml for piperine using 0.5 ml of plasma.

Results: The lowest limit of quantification (LLOQ) for curcumin and piperine was 1.00 and 0.50 ng/ml, respectively. check details The precision of the assay (expressed as coefficient of variation, selleck screening library CV) was < 12.6 % at all concentrations within the standard curve range, with adequate assay accuracy. Stability data revealed that the drugs were stable in plasma under various test conditions.

Conclusion: The method is highly selective and rugged for the estimation of curcumin and piperine in human plasma and would be applicable to toxicokinetic, pharmacokinetic, bioavailability, and bioequivalence studies.”
“Background and Objectives Glucose

and acetate have been proposed to be required elements in platelet storage media. This study investigated the role of these

compounds on the varied elements that comprise the platelet storage lesion. Materials and Methods For each replicate, four pooled and split ABO group-specific buffy coat-derived platelet concentrates were suspended in an in-house additive solution with minimal plasma and varying final concentrations of acetate or glucose. Units were sampled on days 2, 3, 6, 8 and 10 and tested for markers of platelet morphology, activation, function, metabolism and indicators of cell death. Results The absence of glucose was associated with a decrease in ATP, falling to a mean of 1 center dot 1 +/- 0 center dot 1mol/1011 plts in units with no added glucose compared with 4 center dot 2 +/- 0 center dot 6mol/1011 plts (P<0 center dot 001) in units with 30mm glucose. As glucose see more became depleted, the decrease in ATP to levels below 3mol/1011 plts was associated with an increase in both annexin V binding and intracellular free calcium. In units lacking exogenous acetate, ATP levels on day 10 were 5 center dot 2 +/- 1 center dot 5mol/1011 plts compared with 2 center dot 7 +/- 0 center dot 9mol/1011 plts in units with 56mm acetate (P=0 center dot 006). Higher concentrations of exogenous acetate were associated with a lower hypotonic shock response and higher surface expression of CD62P suggestive of a dose dependency.