With this particular in mind, we planned to investigate whether or not the all-natural supplement Cellfood may well have antiproliferative results in vitro, limiting cell proliferation and selling cell death. CF is actually a proprietary formulation containing 78 ionic/colloidal trace components and minerals combined with 34 enzymes and 17 amino acids, all suspended in a resolution of deuterium sulphate. The natural and inorganic elements of the supplement are extracted from the marine red algae Lithothamnion calcareum, whose mineral extract has shown development inhibitory effects on human colon carcinoma cells at the same time as inhibition of liver tumor formation in C57BL6 mice. Referring to CF formulation, former studies have demonstrated its capacity to furnish helpful in vitro anti oxidant safety. At the similar time, the capability of CF to modulate O2 availability and mitochondrial re spiratory metabolism has become evidenced in endothelial cells.
Each one of these observations led us to investigate the poten tial purpose of CF as hypoproliferative experienced agent in vitro. For this goal, we analyzed the impact of CF on cell growth, viability, glycolytic profile, and apoptosis on 3 hu man leukemia cell lines, Jurkat, U937, and K562. Eight een percent of malignancies are of hematological origin, also, leukemic cells are extremely glycolytic, although these cells reside within the bloodstream at higher oxygen tensions than cells in many usual tissue. Inside the current review we reported evidence that CF showed antiproliferative result around the over mentioned leukemia cell lines because of apoptosis induction and tumor metabolism modifications. Methods Cellfood The supplement was kindly presented by Eurodream srl and stored at room temperature. CF was diluted in phosphate buffered sa line and sterilized utilizing a 0.
45 um syringe filter ahead of use. Cell culture Three human leukemia cell lines had been utilized in this examine, Jurkat, U937, and K562. Cells have been grown in RPMI 1640 medium supplemented with 10% heat inactivated fetal bovine serum, 1% L glutamine SCH66336 solubility and 1% penicillin/streptomycin 100 U/ml, and incubated in a CO2 incubator. Cell culture reagents had been from VWR Worldwide. Lymphocytes have been isolated from blood samples pro vided by balanced volunteers by centrifugation inside the presence of Lymphoprep, and have been cultured as described above using the addition of ten ug/ml of phytohemagglutinin. A single dose of CF was administered to leukemia cells or lymphocytes, cells have been collected right after 24, 48, and 72 h of CF administra tion. Untreated cells served as controls. Trypan blue cell counting was performed at every experimental time point to evaluate the viable cell number. Cell viability assay Cell proliferation and viability have been analyzed at 450 nm by the WST one reagent 2 2H five tetrazolio 1,3 benzene disulfonate. The assay was primarily based around the cleavage with the tetrazolium salt WST 1 by mito chondrial dehydrogenases in viable cells.